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互养菌（Syntrophus） 1

互营杆菌（Syntrophorhabdaceae） 1

偃麦草 1

升流式厌氧污泥床（UASB）颗粒污泥 1

多年生 1

小麦 1

苯酚厌氧降解 1

荧光原位杂交 1

隐杆厌氧菌（Cryptanaerobacter） 1

麦草 1

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

 

关键词： Citrus / 2n gamete / interploidy hybridization / pericentromeric SNP marker / second division restitution    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

《农业科学与工程前沿（英文）》   页码 302-313 doi: 10.15302/J-FASE-2021385

摘要： Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

关键词： Citrus     2n gamete     interploidy hybridization     pericentromeric SNP marker     second division restitution    

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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 121-122 doi: 10.15302/J-FASE-2017142

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 48-58 doi: 10.15302/J-FASE-2016114

摘要： Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

关键词： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

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Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

《结构与土木工程前沿（英文）》 2019年 第13卷 第6期   页码 1338-1349 doi: 10.1007/s17009-019-0558-2

摘要： In the present study, steel and polypropylene (PP) fibers have been utilized with the intent of obtaining hybrid fiber-reinforced concrete (HFRC) with desirable mechanical properties. An attempt has been made to scrutinize the properties of HFRC with the main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization in producing synergy. Accordingly, a total of 180 specimens, representing 20 different mixtures have been cast and evaluated through compressive, split tensile, and flexural tests. The relevant flexural toughness of the specimens was calculated using ASTM C1018, ASTM C1609, JSCE, and PCS methods, and the effectiveness of these methods was evaluated based on the experimental results. It was observed that steel fibers are more effective in the improvement of flexural toughness in the presence of PP fibers. Furthermore, synergy associated with the combination of fibers at different stages of deflection of the beam specimens was observed and analyzed.

关键词： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

《医学前沿（英文）》 2023年 第17卷 第3期   页码 493-502 doi: 10.1007/s11684-022-0946-x

摘要： Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

关键词： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry     variant allele frequency     intratumoral heterogeneity     targeted therapy    

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Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

《环境科学与工程前沿（英文）》 2010年 第4卷 第4期   页码 449-458 doi: 10.1007/s11783-010-0250-x

摘要： A maize variety, Huatian-1, had an unusually low translocation rate of cadmium (Cd) (59.6 mg·kg in the roots and 0.093 mg·kg in the grain) compared to 24 other varieties while being grown in soils with 16.50 mg·kg Cd. This indicates that this particular species may have special mechanisms that affect the absorption and translocation pattern of Cd. In this paper, the technique of suppression subtractive hybridization (SSH) was used to isolate and identify Cd-induced genes from Huatian-1 hydroponically ?exposed? to? 0.1 mM ?CdCl ?for? 1 h,? 12 h, 24 h, and 48 h. We found a total of 15 differentially expressed genes in the four groups; 2, 3, 4, and 6 genes were from the groups of 1 h, 12 h, 24 h, and 48 h treatment, respectively. Phospholipase mRNA, adenosine triphosphate (ATP) phosphoribosyl transferase 2, and were turned on in the maize in response to Cd stress, and it might provide new clues to explain the mechanism of maize tolerance to Cd.

关键词： Zea mays L.     cadmium (Cd)     suppression subtractive hybridization    

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Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 615-629 doi: 10.1007/s11705-020-1989-7

摘要： A cholesterol oxidase (COD) was hybridized with Ca , Zn , Al , Fe and Mn . After precipitation with PO at 4 °C for 72 h, the resulting pellets were freeze-dried. In scanning electron microscopy assays, the metal-COD complexes revealed flower-like or granular structures after hybridization. Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials. X-ray diffraction analysis indicated that COD was encapsulated in CaHPO ·2H O-, Zn (PO ) ·4H O-, AlPO -, FeP - and Mn (PO ) ·3H O-based nanostructures, respectively. Differential scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5 °C to 167.02 °C, 167.02 °C, 137.70 °C, 172.85 °C and 160.99 °C, respectively. Using steroid derivatives as substrates, this enzyme could convert cholesterol, pregnenolone, dehydroepiandrosterone, ergosterol, -sitosterol and stigmasterol to related single products. Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme. In addition, substrate selectivity could be affected by various metal materials. Briefly, using Ca , Zn , Al , Fe and Mn as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures, and could help to promote the stability and tolerant properties of the enzyme, while also enhancing its catalytic characteristics.

关键词： cholesterol oxidase     metal-enzyme hybridization     nanostructures     sterol derivatives     bioconversion    

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Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

《环境科学与工程前沿（英文）》 2011年 第5卷 第2期   页码 277-282 doi: 10.1007/s11783-010-0226-x

摘要： Sequencing batch reactor (SBR) for enhanced biological phosphorus removal (EBPR) processes was used to investigate the impact of the temperature shock on the competition between phosphorus-accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in start-up stage. During the 34 days operation, SBR was set with temperature variation(0–5 d, 22±1°C; 6–13 d, 29±1°C; 14–34 d, 14±1°C). PAOs and GAOs were analyzed by fluorescent in situ hybridization (FISH), and intracellular polyphosphate granules were stained by Neisser-stain. The results showed that the influence of temperature shock on PAOs’ abundance was more serious than that on GAOs in the enriching process. Under sudden and substantially temperature variation, from 22±1°C to 29±1°C and then to 14±1°C, the domination of PAOs was deteriorated. After temperature shock, PAOs’ competitive advantages at low temperature that concluded in other study did not appear in our study. As mesophilic, GAOs (indicated by and ) were more temperature adaptive and better grew and took the domination at 14±1°C in the end. In the competition process, organisms of tetrad forming organisms (TFOs)-like shape which were considered as typical GAOs, were observed. With the evidence of poly-P granules containing by Neisser-straining and result of FISH, these organisms of TFOs-like shape were better to be assumed as adaption state or a special self-protecting shape of PAOs.

关键词： fluorescent in situ hybridization (FISH)     tetrad forming organisms (TFOs)     temperature variation     enhanced biological phosphorus removal (EBPR)    

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小麦- 偃麦草远缘杂交选育多年生小麦述评 Review

崔磊,任永康,Timothy D. Murray,闫文泽,郭庆,牛瑜琦,孙玉,李洪杰

《工程（英文）》 2018年 第4卷 第4期   页码 507-513 doi: 10.1016/j.eng.2018.07.003

摘要：

偃麦草属植物（Thinopyrum spp.）是小麦（Triticum aestivum L.）的近缘植物，具有多年生生长习性，并且抗多种生物胁迫和非生物胁迫，可以应用于小麦遗传改良。偃麦草的很多优良性状可通过渐渗育种培育小麦品种。偃麦草的多年生习性是一种由多个未知基因控制的复杂的数量性状。偃麦草属植物可以与普通小麦杂交产生双二倍体或部分双二倍体。此外，通过偃麦草直接驯化也可以选育多年生小麦。小麦- 偃麦草杂种后代结合了双亲的优异性状，可以粮饲兼用。小麦- 偃麦草杂种后代能够适应多种农业生态系统。本文总结了利用偃麦草培育多年生小麦的发展情况，以及小麦-偃麦草杂种的遗传特点、选育方法以及应用前景。

关键词： 偃麦草     麦草     多年生     小麦    

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Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

《环境科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 616-624 doi: 10.1007/s11783-013-0505-4

摘要： The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifying phosphorus (P) removal performance and N O production was examined using a denitrifying enhanced biologic phosphorus removal (EBPR) sludge acclimatized with mixed acetate (HAc) and propionate (Pro) (in the molar ratio 3∶1) as carbon sources. The results showed that when the AnRT was prolonged from 90 to 150 min, the anaerobic polyhydroxyalkanoate (PHA) synthesis was decreased by 15.3%. Moreover, the ineffective PHA consumption occurred in anaerobic phases and contributed to an increased accumulation and higher free nitrous acid (FNA) concentrations (≥0.001–0.0011 mg HNO -N/L) in the subsequent anoxic phases, causing a severe inhibition on anoxic P-uptake and denitrification. Accordingly, the total nitrogen (TN) and total phosphorus (TP) removal efficiencies dropped by approximately 6.3% and 85.5%, respectively; and the ratio of anoxic N O-N production to TN removal increased by approximately 3.8%. The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated by (62.0% (SE = 1.5%)). In conclusion, the short-term excessive anaerobic reaction time negatively impacted denitrifying P removal performance and stimulated more N O production, and its effect on P removal was more obvious than that on nitrogen removal.

关键词： Denitrifying phosphorus removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescence in-situ hybridization    

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基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究 Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

《工程（英文）》 2022年 第9卷 第2期   页码 61-66 doi: 10.1016/j.eng.2021.05.012

摘要：

利用荧光原位杂交（FISH）和克隆文库构建技术，研究了厌氧污泥床反应器处理含酚废水时所收获的颗粒的微生物群落结构。研究发现，互营杆菌（Syntrophorhabdaceae）和隐杆厌氧菌（Cryptanaerobacter）的克隆是苯酚降解的主要原因。为了准确地对隐杆厌氧菌的克隆进行分类归属，需要利用近全长的16S核糖体RNA（rRNA）基因序列进行进化分析。设计了三种寡核苷酸探针用于检测以下三个分类群：互营杆菌、隐杆厌氧菌和互养菌。对厌氧颗粒薄片的FISH 分析显示细菌和古菌呈随机分布。然而，互营杆菌、隐杆厌氧菌和互养菌分布明确。在颗粒细胞的外层发现了隐杆厌氧菌和互养菌，两者关系密切，而互营杆菌则位于颗粒细胞的深层。细菌之间的代谢联系和对底物的亲和力很可能是造成这种特殊分布的原因。研究人员观察到颗粒污泥中苯酚的降解主要通过以下方式进行：首先，隐杆厌氧菌将苯酚转化为苯甲酸盐，然后被互养菌降解为乙酸盐。苯酚的这种互饲降解发生在颗粒表面附近，此处苯酚浓度较高。在苯酚浓度较低的颗粒深部，互营杆菌将苯酚降解为乙酸盐。结果表明，互营杆菌不太可能产生苯甲酸盐，并将其作为中间产物来喂养邻近的生物，这一点与以往研究所提出的理论相矛盾。

关键词： 隐杆厌氧菌（Cryptanaerobacter）     荧光原位杂交     苯酚厌氧降解     互营杆菌（Syntrophorhabdaceae）     互养菌（Syntrophus）     升流式厌氧污泥床（UASB）颗粒污泥    

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标题 作者 时间 类型 操作

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY

期刊论文

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

期刊论文

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

期刊论文

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

期刊论文

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

期刊论文

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

期刊论文

Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

期刊论文

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

期刊论文

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

期刊论文

小麦- 偃麦草远缘杂交选育多年生小麦述评

崔磊,任永康,Timothy D. Murray,闫文泽,郭庆,牛瑜琦,孙玉,李洪杰

期刊论文

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

期刊论文

基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

期刊论文